Formation in Sv4,,-transformed Human Lung Fibroblasts and Biosynthesis in Rat Lung Microsomal Preparations*
نویسندگان
چکیده
Incubation of SV,,-transformed human lung fibroblasts with [“Hlglucosamine for 1 h, followed by chloroform:methanol extraction and thin layer chromatographic analysis, revealed the presence of a major radioactive lipid that was isolated and characterized as GlcUA-(1 + 4)GlcNAc-P-P-dolichol. An identical lipid was formed in smaller quantities under similar incubation conditions in several fibroblastic lines, HeLa cells, and in mouse L cells. Rat lung microsomal preparations catalyze the synthesis of the disaccharide lipid in the following sequence of reactions:
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